Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96

Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage chromatin. Therefore, quality was compared in samples following single-layer centrifugation (SLC) or and controls (extension only) two extenders, INRA82 INRA96. Ejaculates from six stallions were split among treatments: SLC, washing, controls, Sperm motility acrosome status evaluated at 0, 24, 48, 72 96 hours; morphology hours chromatin integrity 0 hours, with storage 6°C. INRA96 had better motility, status, normal than INRA82. The SLC higher fewer reacted acrosomes lower fragmented washed samples. Fewer spermatozoa tail defects observed after washing; those In conclusion, (except for morphology) controls. method is a useful adjunct to preparation, especially before artificial insemination.